Review



generation transfer plasmid plko 1 trc cloning vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Addgene inc generation transfer plasmid plko 1 trc cloning vector
    Generation Transfer Plasmid Plko 1 Trc Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generation+transfer+plasmid+plko+1+trc+cloning+vector/pLKO%2E1+-+TRC+cloning+vector+(Plasmid+%2310878)/bio_rxiv__2024__06__29__601287-313-9-15
    Average 96 stars, based on 1298 article reviews
    generation transfer plasmid plko 1 trc cloning vector - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    shRNA:

    Article Title: Membrane Curvature Promotes ER-PM Contact Formation via Junctophilin-EHD Interactions
    Article Snippet: .. shRNA coding sequences were cloned and inserted into 3rd generation transfer plasmid pLKO.1-TRC cloning vector (Addgene #10878) following the Addgene protocol. .. The sequences of shRNA oligos were either from Sigma-Aldrich’s predesigned shRNA or from Addgene as listed in the plasmid table.

    Article Title: PERK arm of UPR selectively regulates ferroptosis in colon cancer cells by modulating the expression of SLC7A11 (System Xc-)
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer.
    Article Snippet: .. For PERK, SLC7A11, and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein regulates drug resistance and tumorigenic potential via modulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5’CCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG 3’ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: EZH2-H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. Following the same protocol reported in our recent publication EZH2 mouse, KRT14 mouse, and Human shRNA, SP1 mouse, and human and UTX mouse shRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. EZH2 mouse, KRT14 mouse and Human ShRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein promotes drug resistance and tumorigenic potential by inversely regulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5ʹCCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG3ʹ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Clone Assay:

    Article Title: Membrane Curvature Promotes ER-PM Contact Formation via Junctophilin-EHD Interactions
    Article Snippet: .. shRNA coding sequences were cloned and inserted into 3rd generation transfer plasmid pLKO.1-TRC cloning vector (Addgene #10878) following the Addgene protocol. .. The sequences of shRNA oligos were either from Sigma-Aldrich’s predesigned shRNA or from Addgene as listed in the plasmid table.

    Article Title: PERK arm of UPR selectively regulates ferroptosis in colon cancer cells by modulating the expression of SLC7A11 (System Xc-)
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer.
    Article Snippet: .. For PERK, SLC7A11, and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein regulates drug resistance and tumorigenic potential via modulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5’CCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG 3’ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: EZH2-H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. Following the same protocol reported in our recent publication EZH2 mouse, KRT14 mouse, and Human shRNA, SP1 mouse, and human and UTX mouse shRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. EZH2 mouse, KRT14 mouse and Human ShRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein promotes drug resistance and tumorigenic potential by inversely regulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5ʹCCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG3ʹ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Plasmid Preparation:

    Article Title: Membrane Curvature Promotes ER-PM Contact Formation via Junctophilin-EHD Interactions
    Article Snippet: .. shRNA coding sequences were cloned and inserted into 3rd generation transfer plasmid pLKO.1-TRC cloning vector (Addgene #10878) following the Addgene protocol. .. The sequences of shRNA oligos were either from Sigma-Aldrich’s predesigned shRNA or from Addgene as listed in the plasmid table.

    Article Title: PERK arm of UPR selectively regulates ferroptosis in colon cancer cells by modulating the expression of SLC7A11 (System Xc-)
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer.
    Article Snippet: .. For PERK, SLC7A11, and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein regulates drug resistance and tumorigenic potential via modulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5’CCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG 3’ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: EZH2-H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. Following the same protocol reported in our recent publication EZH2 mouse, KRT14 mouse, and Human shRNA, SP1 mouse, and human and UTX mouse shRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. EZH2 mouse, KRT14 mouse and Human ShRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein promotes drug resistance and tumorigenic potential by inversely regulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5ʹCCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG3ʹ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Cloning:

    Article Title: Membrane Curvature Promotes ER-PM Contact Formation via Junctophilin-EHD Interactions
    Article Snippet: .. shRNA coding sequences were cloned and inserted into 3rd generation transfer plasmid pLKO.1-TRC cloning vector (Addgene #10878) following the Addgene protocol. .. The sequences of shRNA oligos were either from Sigma-Aldrich’s predesigned shRNA or from Addgene as listed in the plasmid table.

    Article Title: PERK arm of UPR selectively regulates ferroptosis in colon cancer cells by modulating the expression of SLC7A11 (System Xc-)
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer.
    Article Snippet: .. For PERK, SLC7A11, and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein regulates drug resistance and tumorigenic potential via modulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5’CCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG 3’ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: EZH2-H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. Following the same protocol reported in our recent publication EZH2 mouse, KRT14 mouse, and Human shRNA, SP1 mouse, and human and UTX mouse shRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. EZH2 mouse, KRT14 mouse and Human ShRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein promotes drug resistance and tumorigenic potential by inversely regulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5ʹCCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG3ʹ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Knockdown:

    Article Title: PERK arm of UPR selectively regulates ferroptosis in colon cancer cells by modulating the expression of SLC7A11 (System Xc-)
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer
    Article Snippet: .. For PERK, SLC7A11 , and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: Loss of PERK function promotes ferroptosis by downregulating SLC7A11 (System Xc⁻) in colorectal cancer.
    Article Snippet: .. For PERK, SLC7A11, and ATF4 knockdown generation, shRNA sequences were cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Sequencing:

    Article Title: CXCR4 intracellular protein regulates drug resistance and tumorigenic potential via modulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5’CCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG 3’ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..

    Article Title: EZH2-H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. Following the same protocol reported in our recent publication EZH2 mouse, KRT14 mouse, and Human shRNA, SP1 mouse, and human and UTX mouse shRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis
    Article Snippet: .. EZH2 mouse, KRT14 mouse and Human ShRNA sequence were cloned in the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat # 10878) between unique AgeI and EcoRI restriction sites downstream of the U6 promoter. ..

    Article Title: CXCR4 intracellular protein promotes drug resistance and tumorigenic potential by inversely regulating the expression of Death Receptor 5
    Article Snippet: .. CXCR4 shRNA Sequence: 5ʹCCGGTCCTGTCCTGCTATTGCATTACTCGAGTAATGCAATAGCAGGACAGGATTTTTG3ʹ was cloned into the 3rd generation transfer plasmid pLKO.1 TRC cloning vector (Addgene cat no. 10878) between unique AgeI and EcoRI sites downstream of the U6 promoter. ..



    Similar Products

    96
    Addgene inc generation transfer plasmid plko 1 trc cloning vector
    Generation Transfer Plasmid Plko 1 Trc Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generation+transfer+plasmid+plko+1+trc+cloning+vector/pLKO%2E1+-+TRC+cloning+vector+(Plasmid+%2310878)/bio_rxiv__2024__06__29__601287-313-9-15
    Average 96 stars, based on 1 article reviews
    generation transfer plasmid plko 1 trc cloning vector - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results